Enzymatic synthesis of biodiesel involves the methylation reaction of oil and methanol under the action of lipase at room temperature and pressure. The main advantage of this method is that it does not use any acid-base catalysts at all, and the production process does not produce any waste liquid that has an impact on the environment. The small amount of wastewater generated during the process can meet the discharge requirements with conventional sewage treatment processes; At the same time, the production was carried out completely at near normal temperature (40 ℃) and atmospheric pressure, fundamentally ensuring the essential safety of production, reducing production energy consumption, and saving production costs.